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Macvector drosophila
Macvector drosophila












Reverse genetic techniques are essential tools for researchers in the postgenome project era. Because this Tet-On TA is significantly more efficient than previous ones used in Drosophila, it is also possible to modulate gene induction by controlling the dosage of the antibiotic in the food.

macvector drosophila

In addition, combining the improved Tet-On TA with the Gal4-UAS (upstream-activating sequence) system produces robust, spatially restricted, temporally controlled transgene induction.

macvector drosophila

This TA is superior to all other Tet-On proteins, and its performance is comparable to that of the widely used Tet-Off TA. We report that low levels of doxycycline, when added to normal fly food, efficiently and rapidly induce target transgenes in adults, larvae, and embryos. Here we examine the most promising TA in transgenic Drosophila and characterize its in vivo properties. In HeLa cells, they are far more efficient than the original reverse TA protein, and they exhibit lower background activity in the absence of effectors. Recently, secondgeneration Tet-On transactivators (TAs) have been described. However, use of the Tet-On system has been fraught with difficulties, including high background expression in the absence of effector molecules and inconsistent gene induction. The Tet-Off systems have a number of advantages, including robust target induction using a relatively benign effector molecule.

macvector drosophila

Since their inception, tetracycline (Tet)-inducible systems have become the method of choice for transgenic research.














Macvector drosophila